Quantitative phytochemical analysis of the fungus endophytic extracts isolated from Azadirachta indica using gas chromatography- flame ionization detector

Background information: The phytochemicals of endophytes have generated substantial interest in drug discovery programs because they offer the possibility discovering new biologically active molecules. Objective: objective this study was to quantify and analyze phytochemical profile fungus endophytic extracts isolated from Azadirachta indica leaves, which been used ethnobotanically for treating malaria bacterial infections. Methods: Endophytic fungi were isolated, solid-state fermentation rice medium performed, secondary metabolites extracted according standard techniques. An analytical system that uses gas chromatography flame ionization detection (GC-FID) determine constituents contained endophytes. Results: results GC-FID analysis showed presence Ribalinidine, Naringenin, Sparteine, Phenol, Steroids, Kaempferol, Flavone, Oxalate, Catechin, Tannin, Rutin at different concentrations. Conclusion: This reveals promising ability A. as a foundation naturally occurring bioactive. quantitative assessment endophyte leaves A.indica plant are rich both alkaloids flavonoids (Phenolics).
 Keywords: GC-FID, Phytochemical, Endophytes, Secondary metabolites, Fermentation.